| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Yuko Yoshikawa, Mari Suzuki, Norihiro Yamada and Kenichi Yoshikawa |
| Journal: | FEBS Letters |
| ISSN: | 0014-5793 |
| Volume: | 566 |
| Number: | 1-3 |
| Month: | 5 |
| Year: | 2004 |
| Actual year: | 2004 |
| Pages: | 39-42 |
| doi: | 10.1016/j.febslet.2004.04.008 |
| Abstract: | We have performed a real-time observation of photoinduced breakage on individual long DNA molecules stained by a cyanine dye, YOYO, under the intense illumination (lambda = 450-490 nm) in solution. It was shown that the double-strand breakage is accelerated by Fenton's reagent (Fe2+/H2O2). In addition, it was found that the breakage reaction is protected in the presence of a water-soluble flavonoid, glucosyl-hesperidin. To interpret the kinetic process of the double-strand breakage reaction, a two-step mechanism is proposed: under light illumination on giant DNA molecules, single-strand breaks are generated to induce nicks in a stochastic manner, and then reactive oxygen attacks these nicks causing the double-strand break. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
Yuko Yoshikawa, Mari Suzuki, Norihiro Yamada and Kenichi Yoshikawa, FEBS Letters, 566, 39-42 (2004)