| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Toshio Iwataki, Satoru Kidoaki, Takahiro Sakaue, Kenichi Yoshikawa and Sergey S. Abramchuk |
| Journal: | Journal of Chemical Physics |
| ISSN: | 0021-9606 |
| Volume: | 120 |
| Number: | 8 |
| Month: | 2 |
| Year: | 2004 |
| Actual year: | 2004 |
| Pages: | 4004-4011 |
| doi: | 10.1063/1.1642610 |
| Abstract: | It has been established that in a dilute solution individual giant DNA molecules undergo a large discrete transition between an elongated coil state and a folded compact state. On the other hand, in concentrated solutions, DNA molecules assemble into various characteristic states, including multichain aggregate, liquid crystalline, ionic crystal, etc. In this study, we compared single-chain and multiple-chain events by observing individual chains using fluorescence microscopy. We used spermidine, SPD(3+), as a condensing agent for giant DNA. When the concentration of DNA is below 1 muM in base-pair units, individual DNA molecules exhibit a transition from an elongated state to a compact state. When the concentration of DNA is increased to 10 muM, a thick fiberlike assembly of multiple chains appears. AFM measurements of this thick fiber revealed that more than tens of DNA molecules form a bundle structure with parallel ordering of the chains. The transition between single-chain compaction and bundle formation with multiple-chain assemblies was reproduced by a theoretical calculation. (C) 2004 American Institute of Physics. |
Toshio Iwataki, Satoru Kidoaki, Takahiro Sakaue, Kenichi Yoshikawa and Sergey S. Abramchuk, Journal of Chemical Physics, 120, 4004-4011 (2004)