| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Masatoshi Ichikawa, Hiroki Ichikawa, Kenichi Yoshikawa and Yasuyuki Kimura |
| Journal: | Physical Review Letters |
| ISSN: | 0031-9007 |
| Volume: | 99 |
| Number: | 14 |
| Month: | 10 |
| Year: | 2007 |
| Actual year: | 2007 |
| Pages: | 32767-4 |
| doi: | 10.1103/physrevlett.99.148104 |
| Abstract: | Thermal convection and thermophoresis induced by mu m-scale local heating are shown to elongate a single DNA molecule. An infrared laser used as a point heat source is converged into a dispersion solution of DNA molecules, which is observed under a fluorescent microscope. The thermal convection around the laser focus manifests as extensional flow for the long DNA chain. A simulation of thermal convection that reproduces the experimental condition provides numerical support for the stretching caused by thermal convection. This DNA elongation technique is a novel method for manipulating the intact single DNA molecules, and it can be applied to a lab on a chip. |
Masatoshi Ichikawa, Hiroki Ichikawa, Kenichi Yoshikawa and Yasuyuki Kimura, Physical Review Letters, 99, 32767-4 (2007)