| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Sergey M. Mel'nikov, Vladimir G. Sergeyev, Yulia S. Mel'nikova and Kenichi Yoshikawa |
| Journal: | Journal of the Chemical Society-Faraday Transactions |
| ISSN: | 0956-5000 |
| Volume: | 93 |
| Month: | 1 |
| Year: | 1997 |
| Actual year: | 1997 |
| Pages: | 283-288 |
| doi: | 10.1039/a603753d |
| Abstract: | The interaction between large duplex T4DNA (166 kbp) and a synthetic dialkyl cationic lipid, distearyldimethylammonium bromide (D(18)DAB), was studied using fluorescence microscopy for single-chain observation. The dependence of the higher-order structure of single T4DNAs on the surfactant concentration was evaluated, starting at extremely low values (1.0 x 10(-8) M). We found that individual T4DNA chains undergo a marked discrete transition between elongated coil and compact globule states, and that there is a very wide region of coexistence (about two orders of magnitude of the surfactant concentration) for the coiled and globular T4DNAs. We propose a simple theoretical model for assessing the relationship between the binding equilibrium and the coil-globule transition. In addition, we found that liposomes composed of neutral phospholipids induce unfolding of DNA compacted by a cationic surfactant. |
Sergey M. Mel'nikov, Vladimir G. Sergeyev, Yulia S. Mel'nikova and Kenichi Yoshikawa, Journal of the Chemical Society-Faraday Transactions, 93, 283-288 (1997)