| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Makiko Negishi, Hiroyuki Kitahata and Kenichi Yoshikawa |
| Journal: | Journal of Physical Chemistry B |
| Volume: | 113 |
| Number: | 11 |
| Month: | 3 |
| Year: | 2009 |
| Actual year: | 2009 |
| Pages: | 3264-3268 |
| doi: | 10.1021/jp8113623 |
| Abstract: | The spontaneous generation of a periodic hexagonal superstructure on a giant phospholipid sphere (GPS) with a diameter of 20-200 mu m was studied. The GPS was composed of ternary phospholipids consisting of dioleoylphosphatidylethanolamine (DOPE), dioleoylphosphatidylcholine (DOPC), and dioleoylphosphatidyl-inositol-bisphosphate (DOPIP2). GPSs were prepared by natural swelling of a lipid film formed on a glass substrate. A GPS with a homogeneous lipid mixture tends to form a two-layered structure between the surface and inner parts; the surface layer is attributed to a DOPIP2 rich region (we call this layer SL), and the interior is rich in DOPE and DOPC (we call this layer IL). A hexagonal superstructure develops in the SL, and the topology then changes to form multiple-doughnut structures. Finally, myelin-like tubes are generated through symmetry breaking of the doughnutlike structures. The time-dependent change in the surface-area expansion of a GPS is shown to obey the logistic growth model, and this is attributed to the kinetic process of phase segregation between the surface and bulk phase of the GPS. |
Makiko Negishi, Hiroyuki Kitahata and Kenichi Yoshikawa, Journal of Physical Chemistry B, 113, 3264-3268 (2009)