Publications


Title : Folding Transition of Large DNA Completely Inhibits the Action of a Restriction Endonuclease as Revealed by Single-Chain Observation

Language: English
Type: Article
Authors: Hidehiro Oana, Kanta Tsumoto, Yuko Yoshikawa and Kenichi Yoshikawa
Journal: FEBS Letters
ISSN: 0014-5793
Volume: 530
Number: 1-3
Month: 10
Year: 2002
Actual year: 2002
Pages: 143-146
doi:   10.1016/s0014-5793(02)03448-8
Abstract: The biochemical characteristics of lambda DNA chains in folded/unfolded states upon cleavage by the restriction enzyme ApaLI were investigated in the presence of spermine. These characteristics of DNA chains depending on their higher-order structure were studied at the single-molecule level using fluorescence microscopy. With a low concentration of spermine, lambda DNA takes a random coiled conformation and allows digestion by the enzyme, while under a high concentration of spermine, lambda DNA takes a compact folded structure and inhibits such attack. Together with comparative experiments on short oligomeric DNA, our results suggest that the transition in the higher-order structure causes on/off-type switching of sensitivity to the enzyme. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

"Folding Transition of Large DNA Completely Inhibits the Action of a Restriction Endonuclease as Revealed by Single-Chain Observation"

Hidehiro Oana, Kanta Tsumoto, Yuko Yoshikawa and Kenichi Yoshikawa, FEBS Letters, 530, 143-146, (2002)