| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Sergey V. Mikhailenko, Vladimir G. Sergeyev, Anatoly A. Zinchenko, Marat O. Gallyamov, Igor V. Yaminsky and Kenichi Yoshikawa |
| Journal: | Biomacromolecules |
| ISSN: | 1525-7797 |
| Volume: | 1 |
| Number: | 4 |
| Month: | 12 |
| Year: | 2000 |
| Actual year: | 2000 |
| Pages: | 597-603 |
| doi: | 10.1021/bm0055403 |
| Abstract: | It has been well established that double-stranded DNA undergoes a melting, or helix/coil, transition into a single-stranded coil state with an increase in temperature. On the other hand, it has recently been found that, at a fixed temperature, long DNA, larger than several kilobase pairs, exhibits a discrete transition, or switching, between elongated and folded states, preserving its double-stranded structure, with the addition of various condensation agents, such as alcohol, hydrophilic polymer, multivalent cation, and cationic surfactant. In the present study, we examined the interplay between the folding/unfolding transition and the helix/coil transition in individual giant DNA molecules, by observing the conformation of single molecular chains with fluorescence microscopy. The results indicate that the helix-to-coil transition tightly cooperates with the unfolding transition in DNA. |
Sergey V. Mikhailenko, Vladimir G. Sergeyev, Anatoly A. Zinchenko, Marat O. Gallyamov, Igor V. Yaminsky and Kenichi Yoshikawa, Biomacromolecules, 1, 597-603 (2000)