Publications


Title : Giant DNA molecules exhibit on/off switching of transcriptional activity through conformational transition

Language: English
Type: Article
Authors: Kanta Tsumoto, Luckel Francois and Kenichi Yoshikawa
Journal: Biophysical Chemistry
ISSN: 0301-4622
Volume: 106
Number: 1
Month: 10
Year: 2003
Actual year: 2003
Pages: 23-29
doi:   10.1016/s0301-4622(03)00138-8
Abstract: We found that the transcriptional activity of large DNAs (40 kbp) can be completely inhibited by adding condensing agents, spermine and poly(ethylene glycol), whereas under the same conditions short fragments (140 bp) still show active transcription. Fluorescence microscopic observations of large DNAs revealed clear correlation between the higher-order structure of templates and their transcriptional activity. The steep decrease in transcriptional activity leading to complete inhibition, or on/off switching, is interpreted in terms of conformational transition of the ensemble of DNA molecules.

"Giant DNA molecules exhibit on/off switching of transcriptional activity through conformational transition"

Kanta Tsumoto, Luckel Francois and Kenichi Yoshikawa, Biophysical Chemistry, 106, 23-29, (2003)