| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Hidehiro Oana, Masao Doi, Masanori Ueda and Kenichi Yoshikawa |
| Journal: | Electrophoresis |
| Volume: | 18 |
| Month: | 10 |
| Year: | 1997 |
| Actual year: | 1997 |
| Pages: | 1912-1915 |
| doi: | 10.1002/elps.1150181104 |
| Abstract: | Recently, we found that, in concentrated neutral solutions, DNA molecules migrate in linear conformation under steady electric field. In this paper, we report the conformational change of DNA during 120 degrees crossed-field electrophoresis in the same polymer solution. We found that, in concentrated polyacrylamide solutions, the reorientation process of DNAs becomes simple: the DNA goes back along the previous track and the reorientation time is longer for larger DNA. Such a backtrack motion has been thought to be an essential motion for the separation of DNA fragments in pulsed field gel electrophoresis. We expect that this phenomenon is useful for a more efficient separation technique of large DNAs than the current pulsed field gel electrophoresis. |
Hidehiro Oana, Masao Doi, Masanori Ueda and Kenichi Yoshikawa, Electrophoresis, 18, 1912-1915 (1997)