| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Hidehiro Oana, Koji Kubo, Kenichi Yoshikawa, Haruyuki Atomi and Tadayuki Imanaka |
| Journal: | Applied Physics Letters |
| ISSN: | 0003-6951 |
| Volume: | 85 |
| Number: | 21 |
| Month: | 11 |
| Year: | 2004 |
| Actual year: | 2004 |
| Pages: | 5090-5092 |
| doi: | 10.1063/1.1825064 |
| Abstract: | In this letter, we describe a noninvasive methodology for manipulating single Mb-size whole-genome DNA molecules. Cells were subjected to osmotic shock and the genome DNA released from the burst cells was transferred to a region of higher salt concentration using optical tweezers. The transferred genome DNA exhibits a conformational transition from a compact state into an elongated state, accompanied by the change in its environment. The applicability of optical tweezers to the on-site manipulation of giant genome DNA is suggested, i.e., lab-on-a-plate. (C) 2004 American Institute of Physics. |
Hidehiro Oana, Koji Kubo, Kenichi Yoshikawa, Haruyuki Atomi and Tadayuki Imanaka, Applied Physics Letters, 85, 5090-5092 (2004)