Publications


Title : Controlling the Higher-Order Structure of Giant DNA Molecules

Language: English
Type: Article
Authors: and Kenichi Yoshikawa
Journal: Advanced Drug Delivery Reviews
ISSN: 0169-409X
Volume: 52
Number: 3
Month: 11
Year: 2001
Actual year: 2001
Pages: 235-244
doi:   10.1016/s0169-409x(01)00210-1
Abstract: Giant DNA molecules of size greater than several kbp adopt an elongated coil conformation in aqueous solution. On addition of various kinds of condensing agents, the giant DNA molecules fold into a compact state. With multivalent cations, individual DNA molecules show large discrete transitions between the elongated and compact states. Similarly, with the addition of polymers such as PEG, DNA molecules also switch conformation in an all-or-none manner. In contrast, DNAs fold into a loose compact state with cationic surfactants. The experimental conditions necessary to generate stable soluble particles from single DNA molecules and also to obtain multiple DNA molecular assemblies are outlined. The relationship between the higher-order structure and the efficiency of gene transfection is discussed. (C) 2001 Elsevier Science B.V. All rights reserved.

"Controlling the Higher-Order Structure of Giant DNA Molecules"

and Kenichi Yoshikawa, Advanced Drug Delivery Reviews, 52, 235-244 (2001)