| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Hidehiro Oana, Kanta Tsumoto, Yuko Yoshikawa and Kenichi Yoshikawa |
| Journal: | FEBS Letters |
| ISSN: | 0014-5793 |
| Volume: | 530 |
| Number: | 1-3 |
| Month: | 10 |
| Year: | 2002 |
| Actual year: | 2002 |
| Pages: | 143-146 |
| doi: | 10.1016/s0014-5793(02)03448-8 |
| Abstract: | The biochemical characteristics of lambda DNA chains in folded/unfolded states upon cleavage by the restriction enzyme ApaLI were investigated in the presence of spermine. These characteristics of DNA chains depending on their higher-order structure were studied at the single-molecule level using fluorescence microscopy. With a low concentration of spermine, lambda DNA takes a random coiled conformation and allows digestion by the enzyme, while under a high concentration of spermine, lambda DNA takes a compact folded structure and inhibits such attack. Together with comparative experiments on short oligomeric DNA, our results suggest that the transition in the higher-order structure causes on/off-type switching of sensitivity to the enzyme. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. |
Hidehiro Oana, Kanta Tsumoto, Yuko Yoshikawa and Kenichi Yoshikawa, FEBS Letters, 530, 143-146 (2002)