Publications


Title : Daunomycin Triggers Membrane Blebbing and Breakage of Giant DNA Encapsulated in a Cell-Sized Liposome

Language: English
Type: Article
Authors: Yuko Yoshikawa, Toshio Kanbe and Kenichi Yoshikawa
Journal: Chemical Physics Letters
ISSN: 0009-2614
Volume: 366
Month: 12
Year: 2002
Actual year: 2002
Pages: 305-310
doi:   10.1016/s0009-2614(02)01587-7
Abstract: We studied the effect of daunomycin, a cancer chemotherapeutic agent, on a model cellular system in which folded compact DNA was encapsulated inside a cell-sized phospholipid liposome. Real-time observation with fluorescence microscopy revealed that, upon the addition of daunomycin, entrapped DNA was first elongated and then cut into fragments. A blebbing transition of the membrane was also observed. A theoretical model is proposed to explain the blebbing phenomenon induced by daunomycin, taking into account its effect on the asymmetry between the outer and inner layers of the membrane. (C) 2002 Elsevier Science B.V. All rights reserved.

"Daunomycin Triggers Membrane Blebbing and Breakage of Giant DNA Encapsulated in a Cell-Sized Liposome"

Yuko Yoshikawa, Toshio Kanbe and Kenichi Yoshikawa, Chemical Physics Letters, 366, 305-310 (2002)