Title : All-or-None Folding Transition in Giant Mammalian DNA

Language: English
Type: Article
Authors: Kenichi Yoshikawa, Yuko Yoshikawa and Toshio Kanbe
Journal: Chemical Physics Letters
ISSN: 0009-2614
Volume: 354
Number: 3-4
Month: 3
Year: 2002
Actual year: 2002
Pages: 354-359
doi:   10.1016/s0009-2614(02)00137-9
Abstract: Using fluorescence microscopy, we studied the folding transition induced by spermidine (3+) in individual giant DNAs isolated from pig liver, with an average size of 60 kbp varying between 15 and 150 kbp. We found that individual DNA chains undergo a large discrete transition, or switching, from an elongated coil state to a folded compact state. Interestingly, the width Of the region of coexistence is rather narrow with respect to the spermidine concentration, despite the polydispersity of the DNA specimen. The weak dependence of the manner of the folding transition on molecular weight is discussed based on a simple mean-field theory. (C) 2002 Elsevier Science B.V. All rights reserved.

"All-or-None Folding Transition in Giant Mammalian DNA"

Kenichi Yoshikawa, Yuko Yoshikawa and Toshio Kanbe, Chemical Physics Letters, 354, 354-359 (2002)