| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Yukiko Matsuzawa, Yoshiyuki Koyama, Ken Hirano, Toshio Kanbe, Shinji Katsura, Akira Mizuno and Kenichi Yoshikawa |
| Journal: | Journal of the American Chemical Society |
| ISSN: | 0002-7863 |
| Volume: | 122 |
| Number: | 10 |
| Month: | 3 |
| Year: | 2000 |
| Actual year: | 2000 |
| Pages: | 2200-2205 |
| doi: | 10.1021/ja991457f |
| Abstract: | The present study reports the visualization and optical trapping of an individual submicrometer-sized assembly from synthetic polymers using a laser. The assembly was made of PA (palmitic acid) and PEG-A (amino pendant-containing poly(ethylene glycol)) labeled with FITC (fluorescein-5-isothiocyanate type I) at the ratio of PEG-A:FITC = 10:1. The individual submicrometer-sized assemblies were detected at a concentration 50 times lower than the critical aggregation concentration of the PEG-A/PA assembly expected by surface tension measurement. In addition, submicrometer-sized particles were prepared from individual DNA molecules, T4 DNA (166 kbp), in a poly(ethylene glycol) solution. These synthetic assemblies were successfully trapped and transported using a laser. as well as DNA particles. Finally, laser manipulation is shown to be an effective method to judge whether the pair interaction of submicrometer-sized objects is attractive or repulsive. |
Yukiko Matsuzawa, Yoshiyuki Koyama, Ken Hirano, Toshio Kanbe, Shinji Katsura, Akira Mizuno and Kenichi Yoshikawa, Journal of the American Chemical Society, 122, 2200-2205 (2000)