| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Hiroyuki Mayama, Toshio Iwataki and Kenichi Yoshikawa |
| Journal: | Chemical Physics Letters |
| ISSN: | 0009-2614 |
| Volume: | 318 |
| Number: | 1-3 |
| Month: | 2 |
| Year: | 2000 |
| Actual year: | 2000 |
| Pages: | 113-117 |
| doi: | 10.1016/s0009-2614(00)00004-x |
| Abstract: | To clarify the thermodynamics in the folding phase-transition of single DNA molecules, we studied the effect of temperature on the bimodal distribution of conformation for the ensemble of T4DNA chains (166 kbps) at a fixed concentration of poly(ethylene glycol) using single-chain observation with fluorescence microscopy. From the van't Hoff-type relationship, the entropy change in the unfolding transition is Delta S = + 11.3 k/molecule, and the change in the heat content is Delta H = + 4.77 X 10(-20) J/molecule at T-c = 306 K, where k is the Boltzmann's constant. The positive value of Delta S with the unfolding transition is discussed in relation to the increase in the translational entropy of counterions. (C) 2000 Elsevier Science B.V. All rights reserved. |
Hiroyuki Mayama, Toshio Iwataki and Kenichi Yoshikawa, Chemical Physics Letters, 318, 113-117 (2000)