| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Nobuhiko Emi, Satoru Kidoaki, Kenichi Yoshikawa and Hidehiko Saito |
| Journal: | Biochemical and Biophysical Research Communications |
| ISSN: | 0006-291X |
| Volume: | 231 |
| Month: | 2 |
| Year: | 1997 |
| Actual year: | 1997 |
| Pages: | 421-424 |
| doi: | 10.1006/bbrc.1997.6125 |
| Abstract: | Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, me observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1 similar to 2 mu m order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 mu m. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed. |
Nobuhiko Emi, Satoru Kidoaki, Kenichi Yoshikawa and Hidehiko Saito, Biochemical and Biophysical Research Communications, 231, 421-424 (1997)