Publications


Title : Light Illumination Induces Large Change in the Higher Order Structure of Giant DNAs Complexed with Maltosylated Amino-Pendant Polyethylene Glycol and Sodium Dodecyl Sulfate

Language: English
Type: Article
Authors: Yuko Yoshikawa, Yoshiyuki Koyama and Kenichi Yoshikawa
Journal: Polymer Journal
Volume: 31
Number: 11
Month: 11
Year: 1999
Actual year: 1999
Pages: 1025-1028
doi:   10.1295/polymj.31.1025
Abstract: We have performed single-chain observation by fluorescence microscopy on T4 DNAs complexed with maltosylated amino-pendant polyethylene glycol (mal-PEG-A) in the presence of sodium dodecyl sulfate(SDS). With 0.02mM mal-PEG-A/SDS ([NH]=[SDS]=0.02mM). individual DNAs existed as either a compact globule state or a partially unfolded globule state. Aggregates between multiple number of DNA chains are formed at higher concentrations of mal-PEG-A/SDS. With 1 mM mal-PEG-A/SDS, the DNAs take an elongated coil conformation. It was found that the aggregate with multiple DNAs changes its morphology markedly during the focused illumination of 400-500 nm light. Such a morphological change of the complex has been discussed in relation to the ability of SDS micelle to anchor the DNA fragments induced by the cutting with photo-illumination.

"Light Illumination Induces Large Change in the Higher Order Structure of Giant DNAs Complexed with Maltosylated Amino-Pendant Polyethylene Glycol and Sodium Dodecyl Sulfate"

Yuko Yoshikawa, Yoshiyuki Koyama and Kenichi Yoshikawa, Polymer Journal, 31, 1025-1028 (1999)