| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Satoru Kidoaki and Kenichi Yoshikawa |
| Journal: | Biophysical Chemistry |
| Volume: | 76 |
| Number: | 2 |
| Month: | 2 |
| Year: | 1999 |
| Actual year: | 1999 |
| Pages: | 133-143 |
| doi: | 10.1016/s0301-4622(98)00231-2 |
| Abstract: | To understand the conformational behavior of a giant duplex-DNA chain in a mixed solution with various biopolymers with different state of ionization, the higher-order structure of the DNA chain was analyzed with a fluorescence microscope in the presence of polycations (poly-arginine), polyanions (poly-glutamic acid), and neutral polymers (poly-ethylene glycol) as a model for cellular environment. Concentrated medium with neutral polymer induced the discrete folding transition of the DNA. At the threshold condition for the transition, addition of small amounts of either the polycation or the polyanion caused marked structural changes in the folded DNAs. Based on thermodynamic considerations on the experimental results, profile of free energy of a single giant DNA chain was depicted with respect to the size, or the expansion factor or, in the three-dimensional structure of the DNA. The effect of the neural crowding polymer on the degree of folding of a single giant DNA chain is discussed in a semi-quantitative manner. (C) 1999 Elsevier Science B.V. All rights reserved. |
Satoru Kidoaki and Kenichi Yoshikawa, Biophysical Chemistry, 76, 133-143 (1999)