Publications


Title : Entrapping Desired Amounts of Actin Filaments and Molecular Motor Proteins in Giant Liposomes

Language: English
Type: Article
Authors: Kingo Takiguchi, Ayako Yamada, Makiko Negishi, Yohko Tanaka-Takiguchi and Kenichi Yoshikawa
Journal: Langmuir
ISSN: 0743-7463
Volume: 24
Number: 20
Month: 10
Year: 2008
Actual year: 2008
Pages: 11323-11326
doi:   10.1021/la802031n
Abstract: We have successfully prepared cell-sized giant liposomes encapsulating desired amounts of actoHMM, a mixture of actin filament (F-actin) and heavy meromyosin (HMM, an actin-related molecular motor), in the presence of 5 mM MgCl2 and 50 mM KCl. We employed a spontaneous transfer method to prepare those liposomes. In the absence of HMM, F-actin was distributed homogeneously inside the liposomes. In contrast, when F-actin was encapsulated in liposomes together with HMM, network structures were generated. Such network structures are attributable to the cross-linking of F-actin by HMM.

"Entrapping Desired Amounts of Actin Filaments and Molecular Motor Proteins in Giant Liposomes"

Kingo Takiguchi, Ayako Yamada, Makiko Negishi, Yohko Tanaka-Takiguchi and Kenichi Yoshikawa, Langmuir, 24, 11323-11326 (2008)