| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Kingo Takiguchi, Ayako Yamada, Makiko Negishi, Yohko Tanaka-Takiguchi and Kenichi Yoshikawa |
| Journal: | Langmuir |
| ISSN: | 0743-7463 |
| Volume: | 24 |
| Number: | 20 |
| Month: | 10 |
| Year: | 2008 |
| Actual year: | 2008 |
| Pages: | 11323-11326 |
| doi: | 10.1021/la802031n |
| Abstract: | We have successfully prepared cell-sized giant liposomes encapsulating desired amounts of actoHMM, a mixture of actin filament (F-actin) and heavy meromyosin (HMM, an actin-related molecular motor), in the presence of 5 mM MgCl2 and 50 mM KCl. We employed a spontaneous transfer method to prepare those liposomes. In the absence of HMM, F-actin was distributed homogeneously inside the liposomes. In contrast, when F-actin was encapsulated in liposomes together with HMM, network structures were generated. Such network structures are attributable to the cross-linking of F-actin by HMM. |
Kingo Takiguchi, Ayako Yamada, Makiko Negishi, Yohko Tanaka-Takiguchi and Kenichi Yoshikawa, Langmuir, 24, 11323-11326 (2008)