Publications


Title : Phase Separation on a Phospholipid Membrane Inducing a Characteristic Localization of DNA Accompanied by Its Structural Transition

Language: English
Type: Article
Authors: Ayako Kato, Akihiko Tsuji, Miho Yanagisawa, Daisuke Saeki, Kazuhiko Juni, Yasunori Morimoto and Kenichi Yoshikawa
Journal: Journal of Physical Chemistry Letters
Volume: 1
Number: 23
Month: 12
Year: 2010
Actual year: 2010
Pages: 3391-3395
URL:   http://pubs.acs.org/doi/abs/10.1021/jz101376m
doi:   10.1021/jz101376m
Keywords: giant unilamellar vesicle; liposome; membrane domain; phosphatidylethanolamine; DNA−phospholipid interaction; conformational transition of DNA
Abstract: We report the coupling of phase separation on a phospholipid membrane with a structural transition of giant DNA. We performed single-DNA measurements in the presence of cell-sized giant unilamellar vesicles (GUV) composed of dioleoylphosphatidylcholine (DOPC), phosphatidylethanolamine (PE), and cholesterol. We observed the effect of phase separation of the membrane by changing PE from dioleoylphosphatidylethanolamine (DOPE) to dipalmitoylethanolamine (DPPE), which corresponds to a change from a homogeneous single phase to two segregated phases of liquid-ordered and liquid-disordered states on the membrane. In the homogeneous membrane, DNA molecules exhibited a compact conformation in aqueous solution containing a physiological concentration of Mg2+ and polyamine, without attaching to the membrane surface. In contrast, in the membrane that was segregated into two domains, DNA molecules were adsorbed onto the liquid-ordered phase that was rich in DPPE by taking an elongated conformation.

"Phase Separation on a Phospholipid Membrane Inducing a Characteristic Localization of DNA Accompanied by Its Structural Transition"

Ayako Kato, Akihiko Tsuji, Miho Yanagisawa, Daisuke Saeki, Kazuhiko Juni, Yasunori Morimoto and Kenichi Yoshikawa, Journal of Physical Chemistry Letters, 1, 3391-3395 (2010)