Publications


Title : Intercalating Fluorescence Dye YOYO-1 Prevents the Folding Transition in Giant Duplex DNA

Language: English
Type: Article
Authors: Natsuhiko Yoshinaga, Tatsuo Akitaya and Kenichi Yoshikawa
Journal: Biochemical and Biophysical Research Communications
ISSN: 0006-291X
Volume: 286
Number: 2
Month: 8
Year: 2001
Actual year: 2001
Pages: 264-267
doi:   10.1006/bbrc.2001.5376
Abstract: Recently, it has become clear that with the addition of polyamines, giant DNA molecules of size greater than 10 kbp exhibit all-or-none switching between elongated coil and folded compact states. Here the effects of the intercalating fluorescent labeling dye, YOYO-1, and the minor-groove binding fluorescent labeling dye, DAPI, on the folding transition of single giant T4 DNA (166 kbp) induced by spermidine(3+) were examined, by use of the experimental technique of single molecular chain observation with fluorescence microscopy. It is found that the intercalating dye, YOYO-1, markedly prevents the folding transition, whereas the minor-groove binding dye, DAPI, exhibits negligible effect on the folding transition. This action of YOYO-1 is discussed in relation to the biological effect of intercalators.

"Intercalating Fluorescence Dye YOYO-1 Prevents the Folding Transition in Giant Duplex DNA"

Natsuhiko Yoshinaga, Tatsuo Akitaya and Kenichi Yoshikawa, Biochemical and Biophysical Research Communications, 286, 264-267 (2001)