| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Natsuhiko Yoshinaga, Tatsuo Akitaya and Kenichi Yoshikawa |
| Journal: | Biochemical and Biophysical Research Communications |
| ISSN: | 0006-291X |
| Volume: | 286 |
| Number: | 2 |
| Month: | 8 |
| Year: | 2001 |
| Actual year: | 2001 |
| Pages: | 264-267 |
| doi: | 10.1006/bbrc.2001.5376 |
| Abstract: | Recently, it has become clear that with the addition of polyamines, giant DNA molecules of size greater than 10 kbp exhibit all-or-none switching between elongated coil and folded compact states. Here the effects of the intercalating fluorescent labeling dye, YOYO-1, and the minor-groove binding fluorescent labeling dye, DAPI, on the folding transition of single giant T4 DNA (166 kbp) induced by spermidine(3+) were examined, by use of the experimental technique of single molecular chain observation with fluorescence microscopy. It is found that the intercalating dye, YOYO-1, markedly prevents the folding transition, whereas the minor-groove binding dye, DAPI, exhibits negligible effect on the folding transition. This action of YOYO-1 is discussed in relation to the biological effect of intercalators. |
Natsuhiko Yoshinaga, Tatsuo Akitaya and Kenichi Yoshikawa, Biochemical and Biophysical Research Communications, 286, 264-267 (2001)