| Language: | English |
|---|---|
| Type: | Article |
| Authors: | Masahiko Hase, Ayako Yamada, Tsutomu Hamada, Damien Baigl and Kenichi Yoshikawa |
| Journal: | Langmuir |
| ISSN: | 0743-7463 |
| Volume: | 23 |
| Number: | 2 |
| Month: | 1 |
| Year: | 2007 |
| Actual year: | 2007 |
| Pages: | 348-352 |
| doi: | 10.1021/la0618521 |
| Abstract: | Cell-sized water droplets coated by a phospholipid layer mimicking the inner surface of living cells were manipulated by laser tweezers and used as biochemical microreactors. The cell-sized phospholipid-coated microdroplets (CPMDs) consisted of a water droplet in mineral oil with a diameter of 1-100 mu m and coated by 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine. We monitored the time development of biochemical reactions in a single CPMD obtained after the controlled fusion of two CPMDs containing a substrate and an enzyme, respectively. We present results on two enzymatic reactions: calcein production in the presence of esterase and green fluorescence protein expression. |
Masahiko Hase, Ayako Yamada, Tsutomu Hamada, Damien Baigl and Kenichi Yoshikawa, Langmuir, 23, 348-352 (2007)